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Genetic Control of Oxidative Mutagenesis in Sulfolobus acidocaldarius [Article]

July 27, 2020 0

Summary: To identify DNA oxidation defenses of hyperthermophilic archaea, we deleted genes encoding the putative 7,8-dihydro-8-oxoguanine (oxoG)-targeted N-glycosylase of Sulfolobus acidocaldarius (ogg; Saci_01367), the Y family DNA polymerase (dbh; Saci_0554), or both and measured the effects on cellular survival, replication accuracy, and oxoG bypass in vivo. Spontaneous G·C-to-T·A transversions were […]

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The C Terminus of Substrates Is Critical but Not Sufficient for Their Degradation by the Pseudomonas aeruginosa CtpA Protease [Article]

July 27, 2020 0

Summary: Bacterial carboxyl-terminal processing proteases (CTPs) are widely conserved and have been linked to important processes, including signal transduction, cell wall metabolism, and virulence. However, the features that target proteins for CTP-dependent cleavage are unclear. Studies of the Escherichia coli CTP Prc suggested that it cleaves proteins with nonpolar and/or […]